Quick User Guide
Recent Version:MeroX 2.0
(StavroX included)
To test it you can use the test-files
delivered (test.fasta, test.mgf and test.ssf)
- Load a Fasta file containing the aminoacid sequence
by clicking "Load Fasta"-Button. The file name and directory are
shown.
- Load your Mass-Spec data in *.mgf, *.pkl, *.mzXML or *.mzML format.
Again, the file name is shown.
- You can change the settings of your analysis concerning Proteolysis,
Modifications, Neutral Losses, Cross-Linker, Accuracies and Mass
Limits, by clicking the "Settings"-Button.
Start
Window
- After clicking the "Start"-Button, a window pops up
that summarizes the settings used for calculation. If those settings
are ok, use the "OK"-Button to start analysis. Depending on the
sizes of the MS-Data file and Fasta-file as well as the settings used,
the analysis takes several seconds to minutes.
- When the analysis has finished, 2 windows will pop up: 1.)
the Main Analysis Window, showing all identified cross link
candidates 2.) the Decoy Window. The decoy analysis gives an
idea of the quality of the analysis.
Main
Analysis Window
Decoy
Window
- In the Main Analysis Window, select a candidate from
the table in th upper panel. This will show details of this
candidate in the lower 2 panels. On the left is a table giving the different
sites of cross linking that are possible between the 2 peptides and
a schematic representation of the fragmentation of the selected
precursor. The right panel shows the labeled spectrum. By
clicking on the "Details of Crosslink"-Button another window
will open, that shows all necessary information to evaluate a
correct cross-link.
Detail Window